Composite
Part:BBa_K4762022:Design
Designed by: Xuan Hang Group: iGEM23_NWU-CHINA-A (2023-10-11)
T5_Promoter-RBS-CAT-RBS-SOD-RBS-Elafin-T7_Terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1286
Illegal XbaI site found at 2337
Illegal PstI site found at 1119 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1286
Illegal NheI site found at 1101
Illegal NheI site found at 2634
Illegal PstI site found at 1119 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1286
Illegal BamHI site found at 83
Illegal BamHI site found at 1536 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1286
Illegal XbaI site found at 2337
Illegal PstI site found at 1119 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1286
Illegal XbaI site found at 2337
Illegal PstI site found at 1119
Illegal AgeI site found at 153
Illegal AgeI site found at 1943 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon-optimized to work in E. coli
Source
The sequence is synthesized by the company